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This paper presents a signal processing approach to analyse and identify accent discriminative features of four groups of English as a second language (ESL) speakers, including Chinese, Indian, Japanese, and Korean. The features used for speech recognition include pitch, stress, formant frequencies, the Mel frequency coefficient, log frequency coefficient, and the intensity and duration of vowels spoken. This paper presents our study using the Matlab Speech Analysis Toolbox, and highlights how data processing can be automated and results visualised. The proposed algorithm achieved an average success rate of 57.3% in identifying vowels spoken in a speech by the four non-native English speaker groups.